clary sage oil

What is clary sage

Clary sage (Salvia sclarea), is a biennial or short-lived herb in the genus Salvia. It is native to the northern Mediterranean Basin, along with some areas in north Africa and Central Asia. The plant has a lengthy history as a medicinal herb, and is currently grown for its essential oil 1). The distilled clary sage essential oil is used widely in perfumes and as a muscatel flavoring for vermouths (red or white wine flavoured with aromatic herbs), wines, and liqueurs 2). It is also used in aromatherapy 3).

Clary sage (Salvia sclarea) is an important aromatic plant of the mint family that has various pharmacological properties, including antioxidant and antimicrobial activities 4). Blended essential oils containing clary sage, lavender and marjoram have been found to relieve pain in women with dysmenorrhea (painful periods) 5). In rats, clary sage has anti-depressant activity through modulation of the dopaminergic pathway 6). Despite its widespread use in aromatherapy and its therapeutic activities, little is known about the mechanisms of action of clary sage.

Clary sage extract, Sclareol, that is commonly used in perfumery and as a flavoring agent in people’s diets in China and the Mediterranean area has been shown in test tube can inhibit the growth of tumor but not that of the normal cells 7). Sclareol has low toxicity and slows down the tumor growth in vivo (mice study) and diverts the  immune response towards Th1 by increasing the level of IFN-γ and decreasing the level of IL-4 as well as modulating the T regulatory cells. These parameters make Sclareol a candidate to be used as chemoimmunotherapy of cancer 8).

Clary sage oil benefits

Constituents of Salvia sclarea oil

Detailed analysis of the clary sage oil showed 56 constituents, out of which linalyl acetate (57.9%) and linalool (12.4%) were determined as the main ones 9). Moreover, α-pinene (4.5%), α-terpineol (3.5%), sabinene (3.3%), β-pinene (3.0%), geranyl acetate (1.6%), myrcene (1.5%) and neryl acetate (1.0%) were identified in large quantities (see Table 1).

Table 1. Clary sage essential oil constituents

NumberCompound%RI
1α-Thujene/Tricyclene0.3923
2α-Pinene4.5931
3Camphenetr941
4Sabinene3.3966
5β-Pinene3.0970
6Myrcene1.5983
7α-Phellandrene0.1996
8Car-3-ene0.21004
9α-Terpinene0.41009
10p-Cymene0.51012
111,8-Cineole0.81020
12Limonene0.91021
13(Z)-β-Ocimene0.11028
14(E)-β-Ocimene0.21038
15γ-Terpinene0.51049
16trans-Sabinene hydrate0.11054
17trans-Linalool oxide (f)tr1059
18cis-Linalool oxide (f)tr1073
19Terpinolene0.31079
20Linalool12.41090
21cis-Sabinene hydratetr1099
22cis-p-Menth-2-en-1-oltr1108
23Borneoltr1150
24Terpinen-4-ol0.91163
25α-Terpineol3.51175
26γ-Terpineol0.21181
27Linalyl acetate0.11200
28Nerol0.61216
29Ascaridole0.11221
30Linalyl acetate57.91249
31Safrole0.21268
32α-Terpinyl acetate0.11334
33Neryl acetate1.01344
34α-Cubebenetr1349
35Geranyl acetate1.61363
36α-Copaenetr1371
37β-Bourbonene0.21377
38β-Cubebene0.11385
39β-Elemene0.11389
40β-Caryophyllene0.81420
41β-Copaenetr1428
42trans-α-Bergamotenetr1433
43(E)-β-Farnesenetr1447
44α-Humulene0.11452
45Germacrene D0.51477
46β-Selinenetr1483
47Myristicin1.61492
48δ-Cadinene0.11514
49Elemicintr1520
50Spathulenoltr1565
51Caryophyllene oxide0.11571
52β-Eudesmoltr1625
53α-Eudesmoltr1637
54Sclareoloxidetr1881
55Geranyllinalooltr1906
56Sclareol0.12206

tr < 0.05%, % – percentage of constituents, RI – retention index.

[Source 10)]

This study 11) showed that the clary sage essential oil has the strong anti-staphylococcal activity against clinical strains isolated from wound infections. Interestingly, the strains of species Staphylococcus aureus and Staphylococcus epidermidis were more susceptible to the clary sage oil, followed by strains of Staphylococcus xylosus. Staphylococcus aureus strains including MRSA (methicillin-resistant Staphylococcus aureus – the most resistant staph bacteria that’s become resistant to many of the antibiotics used to treat ordinary staph infections) 12). Linalyl acetate (57.9%) and linalool (12.4%) were found to be the main compounds out of 56 constituents of the clary sage oil.

Clary sage oil has been found to contain significant levels of the psychoactive monoterpane linalool 13). In a study on experimental mice, where the mice received oil supplemented food from the age of 4 weeks or from conception via their pregnant dams. Each age group received either clary sage oil– or sunflower oil–enriched feed. Dominant animals, whose pregnant mothers received clary sage oil–enriched feed from the date of conception, showed a significant reduction of dominant and anxiety-like behavior, in comparison to their sunflower oil–treated counterparts. Clary sage oil–treated submissive animals exhibited a similar tendency, and showed a significant reduction in blood corticosterone levels. These findings support the hypothesis that clary sage oil possesses anxiolytic properties 14).

In another rats study subjected to immobilization stress, clary sage treatment of rats contributed in recovery from endothelial dysfunction by decreasing oxidative stress and increasing nitric oxide production and endothelial nitric oxide synthase expression 15). Endothelial dysfunction in hypertensive patients has also been associated with decreased production of nitric oxide, a key vasodilator released by the endothelium. Nitric oxide has been associated with various endothelial functions, including the regulation of vascular tone, platelet aggregation, and vascular smooth muscle cell proliferation 16). Therefore, endothelial dysfunction may result from the reduced activity of endothelial nitric oxide synthase and the resulting decreased bioavailability of nitric oxide 17).

Clary oil was also found to alleviate stress and have antidepressive effects in a mouse model, effects manifested by activation of paths with dopamine characteristics 18). In addition, inhalation of clary oil vapors for 20 minutes by patients with gingivitis was found to reduce the strain and stress of patients undergoing dental treatment 19).

In a human study 20) examining the effects of inhalation of clary oil or lavender oil vapors (both of which contain high concentrations of linalyl acetate) at concentrations of 5%, involving women who underwent urodynamic assessments for urinary incontinence. The clary oil group experienced a significant decrease in systolic blood pressure compared with the control (almond oil) and lavender oil groups, a significant decrease in diastolic blood pressure compared with the lavender oil group and a significant decrease in respiratory rate compared with the control group. This study showed clary oil inhalation may be useful in inducing relaxation in female urinary incontinence patients undergoing urodynamic assessments 21).

Clary sage extracts and essential oil possess antioxidant and antimicrobial properties 22). Dźamić et al. 23) showed fungicidal activity of the sage oil against Aspergillus, Penicillium, Fusarium, Trichoderma, Mucor, Candida and also its fungistatic and fungicidal activity against Cladosporium, Trichophyton, Alternaria and Phoma in very low levels. Hristova et al. 24) presented interesting results connected with high activity of the clary sage oil against many clinical strains of Candida species.

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